Single-channel dose-response studies in single, cell-attached patches
نویسندگان
چکیده
منابع مشابه
Dual-pore glass chips for cell-attached single-channel recordings.
While high-throughput planar patch-clamp instruments are now established to perform whole-cell recordings for drug screening, the conventional micropipette-based approach remains the gold standard for performing cell-attached single-channel recordings. Generally, planar platforms are not well-suited for such studies due to excess noise resulting from low seal resistances and the use of substrat...
متن کاملSingle channel currents from excised patches of muscle membrane.
The currents through single acetylcholine-activated channels were measured on membrane fragments that had been torn from rat muscle myotubes with patch pipettes. The membrane fragments were sealed into the pipette by using the "gigohm-seal" technique of Neher, which also permitted voltage clamp of the membrane via the patch electrode. Membrane patches were excited by sudden withdrawal of the el...
متن کاملA comparison of calcium-activated potassium channel currents in cell- attached and excised patches
Single channel currents from Ca-activated K channels were recorded from cell-attached patches, which were then excised from 1321N1 human astrocytoma cells. Cells were depolarized with K (110 mM) so that the membrane potential was known in both patch configurations, and the Ca ionophore A23187 or ionomycin (20-100 microM) was used to equilibrate intracellular and extracellular [Ca] (0.3 or 1 mic...
متن کاملParticulate Contamination in Single-Dose Parenteral Antibiotics in Iran
In order to ensure the safety of parenterals, international pharmacopoeias and national standards have set up stringent guidelines and standards. Particulate con- tamination is a potential health risk caused by intravenous injection of particles large enough to potentially clog the small arteries. Particles could be produced through manufacturing and packaging or even dispensing of the ph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1991
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(91)82095-1